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5% commercial hypochlorite and benzalconium chloride quaternary Epicoccum sp. with an identification
70% ethanol, were cultivated in PDA ammonium 200g/L and metiram 700 percentage of 99.0%. Susceptibility
Agar for 7-15 days at 28°C, purified by g/kg, diluted at concentrations 50% tests identified that pesticides with
microculture and monosporic culture, above and below those recommended the active substance Difeconazole
and molecularly identified by PCR by the manufacturer, in PDA plates, 250g/L were the most effective,
using STI NDAs feeders, both STI1 F with pre-drilled wells, adding 80µL of having a fungistatic action, where at
and STI4 R (Abata, 2017; Guarín Torres each treatment and the witness (sterile low concentrations (50% less than the
et al. 2019; Umesha et al. 2016). distilled water), the microorganism recommended concentration) inhibited
The agar diffusion methodology for was centrally inoculated in the the growth of these phytopathogenic
susceptibility testing using pesticides medium and incubated for 7-15 days fungi up to 62.5% on average, compared
with active substance was carried out: at 28°C (Bartlewicz et al. 2016). The to high concentrations (50% more
validamicin 30g/L, Iodine 132.0g/L, identified fungal genera corresponded concentrations) where they inhibited
methyl-2-benzimidazole carbamate to Alternaria alternata with an up to 65.0%, on average.
500g/L, difeconazole 250g/L, identification percentage of 99.5% and

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